In vivo and in vitro toxicity of phospholipase C from Pseudomonas aeruginosa

Toxicon. 1992 Feb;30(2):161-9. doi: 10.1016/0041-0101(92)90469-l.

Abstract

Pseudomonas aeruginosa produces phospholipase C (PLC), a heat-labile hemolysin. Histopathological analysis of PLC-treated mice revealed that the primary target organs involved in PLC-induced toxicity were the liver and kidney. Mice treated i.v. with PLC demonstrated significant tubular epithelial necrosis of the kidney with hematuria, while when given i.p. they exhibited hepatonecrosis with cellular infiltration. Splenomegaly was also a consistent finding. Results from in vitro studies indicate that PLC is toxic for mouse peritoneal cells and human leukocytes.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Bacterial Toxins / metabolism
  • Bacterial Toxins / toxicity*
  • Heart / drug effects
  • Humans
  • In Vitro Techniques
  • Kidney / drug effects
  • Kidney / pathology
  • Leukocytes, Mononuclear / metabolism
  • Liver / drug effects
  • Liver / pathology
  • Lung / drug effects
  • Lung / pathology
  • Mice
  • Mice, Inbred C3H
  • Myocardium / pathology
  • Neutrophils / drug effects
  • Neutrophils / ultrastructure
  • Peritoneal Cavity / cytology
  • Pseudomonas aeruginosa / enzymology*
  • Spleen / drug effects
  • Spleen / pathology
  • Type C Phospholipases / metabolism
  • Type C Phospholipases / toxicity*

Substances

  • Bacterial Toxins
  • Type C Phospholipases