[Relationship of APOE gene polymorphism with subclasses of serum high density lipoprotein in hyperlipidemia]

Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2004 Dec;21(6):615-8.
[Article in Chinese]

Abstract

Objective: To investigate apolipoprotein E(apoE) polymorphism and its relationship with serum lipids and apolipoprotein, serum high density lipoprotein (HDL) subclasses in patients with hyperlipidemia(HL).

Methods: APOE genotype was assayed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The subclasses of serum HDL in 112 patients with hyperlipidemia and 73 healthy subjects were determined by two-dimensional gel electrophoresis in conjunction with immunodetection method.

Results: APOE3/3 genotypes and allele epsilon3 frequency in HL group and control group were both the highest. In HL group, the genotype of APOE2 had higher serum APOE/CIII ratio and lower HDL3b levels, compared with the genotype of APOE3 (P<0.05). In control group, the genotype of apoE2 had higher serum triglycerides, APOE levels and APOE/CIII ratio, compared with the genotype of APOE3 and APOE4 (P<0.05).

Conclusion: Polymorphism of APOE gene may relate to the distribution of HDL particles.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Apolipoprotein E2
  • Apolipoprotein E3
  • Apolipoproteins E / blood
  • Apolipoproteins E / genetics*
  • Cholesterol / blood
  • Female
  • Gene Frequency
  • Genotype
  • Humans
  • Hyperlipidemias / blood
  • Hyperlipidemias / genetics*
  • Lipoproteins, HDL / blood*
  • Lipoproteins, HDL / classification
  • Male
  • Middle Aged
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Triglycerides / blood

Substances

  • Apolipoprotein E2
  • Apolipoprotein E3
  • Apolipoproteins E
  • Lipoproteins, HDL
  • Triglycerides
  • Cholesterol