Heat sensitivity of a SARS-associated coronavirus introduced into plasma products

Vox Sang. 2004 Nov;87(4):302-3. doi: 10.1111/j.1423-0410.2004.00577.x.


Background and objectives: Various measures to inactivate/remove viruses have been implemented for manufacturing plasma-derived products. Here, we examined the heat inactivation ability of an agent of the severe acute respiratory syndrome (SARS), SARS coronavirus (CoV).

Materials and methods: The Frankfurt-1 strain of SARS-CoV was incorporated in manufacturing processes of several products by using samples collected immediately before liquid heat treatment at 60 degrees C.

Results: SARS-CoV was easily inactivated by this treatment for 60 min in all in-process samples. However, the different composition of the tested samples affected the heat sensitivity of the virus strain: the infectivity of the virus in Antithrombin III preparation still remained after heating for 30 min at 60 degrees C.

Conclusion: If by rare chance SARS-CoV contaminates source plasma, there should be no or only minor risk of this virus infection, due to sufficient inactivation by the 60 degrees C 10 h liquid heating step, although we must pay attention to the composition used for blood product preparation.

MeSH terms

  • Blood / virology*
  • Blood Transfusion / methods
  • Blood Transfusion / standards
  • Consumer Product Safety
  • Hot Temperature*
  • Humans
  • Kinetics
  • Severe acute respiratory syndrome-related coronavirus*
  • Sterilization / methods*
  • Virus Inactivation*