PKCdelta is involved in signal attenuation in CD3+ T cells

Immunol Lett. 2005 Jan 31;96(2):291-3. doi: 10.1016/j.imlet.2004.08.011.

Abstract

PKCdelta has been implicated in the signalling events after engagement of the antigen specific receptor on B cells and the Fc-epsilon receptor on mast cells. Employing our recently established PKCdelta null mice , we here investigate the physiological function of PKCdelta in CD3+ T cells. As result, administration of anti-CD3 antibodies in vivo induced markedly increased blood plasma IL-2 levels (but not IL-4, IFN-gamma, TNF-alpha and IL-6 levels) in the PKCdelta null mice, when compared to wild-type sibling controls. Additionally, in vitro T cell proliferative responses to allogenic MHC are significantly enhanced in PKCdelta-deficient CD3+ T cells. These findings suggest that PKCdelta serves a so far unrecognized role in TCR-induced negative regulation of IL-2 cytokine production and T cell proliferation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / administration & dosage
  • CD3 Complex / analysis*
  • CD3 Complex / immunology
  • Interleukin-4 / blood
  • Interleukin-4 / metabolism
  • Lymphocyte Activation / physiology*
  • Mice
  • Mice, Mutant Strains
  • Protein Kinase C / genetics
  • Protein Kinase C / physiology*
  • Protein Kinase C-delta
  • Receptors, Antigen, T-Cell / physiology*
  • Signal Transduction
  • T-Lymphocytes / enzymology*
  • T-Lymphocytes / immunology*

Substances

  • Antibodies
  • CD3 Complex
  • Receptors, Antigen, T-Cell
  • Interleukin-4
  • Prkcd protein, mouse
  • Protein Kinase C
  • Protein Kinase C-delta