Generation of clinical-grade monocyte-derived dendritic cells using the CliniMACS system

Methods Mol Med. 2005;109:71-82. doi: 10.1385/1-59259-862-5:071.

Abstract

We describe the generation of monocyte-derived dendritic cells (MoDC) from leukapheresis products using the CliniMACS system from Miltenyi Biotec. In a clinical setting, the method turned out to be feasible for the generation of clinical-grade MoDC from patients with metastatic renal-cell carcinoma. MoDC generated with this system exhibited a fully mature phenotype as well as high migratory and T-cell stimulatory capacity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Differentiation
  • Cell Movement
  • Cell Separation / methods*
  • Dendritic Cells / cytology*
  • Dendritic Cells / immunology
  • Flow Cytometry
  • Humans
  • Immunomagnetic Separation / methods*
  • Leukapheresis
  • Lymphocyte Culture Test, Mixed
  • Monocytes / cytology*
  • Monocytes / immunology
  • Receptors, CCR7
  • Receptors, Chemokine / metabolism

Substances

  • CCR7 protein, human
  • Receptors, CCR7
  • Receptors, Chemokine