[Expression of telomere repeat binding factor 1 (TRF1) protein in kidney cancer]

Zhejiang Da Xue Xue Bao Yi Xue Ban. 2004 Nov;33(6):496-9, 508. doi: 10.3785/j.issn.1008-9292.2004.06.006.
[Article in Chinese]

Abstract

Objective: To investigate the expression levels of telomere repeat binding factor 1(TRF1) protein in normal kidney tissue and kidney cancer.

Methods: Specimens of kidney cancer and pericancerous tissues were collected from 32 cases of renal carcinoma. A quantitative Western blotting technique was developed using TRF1 monoclonal antibody to determine the expression level of TRF1 protein in total protein extracts from tissue specimens.

Results: The expression level of TRF1 protein was higher in normal kidney tissues (3.611 +/-1.922 microg/microl) than that of cancer tissues (2.428 +/-1.352 microg/microl) (t=5.776, P<0.01).

Conclusion: The expression level of TRF1 protein is significantly reduced in kidney cancer and the level is negatively correlated with malignant degree of the cancer.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma, Clear Cell / metabolism
  • Adult
  • Aged
  • Aged, 80 and over
  • Female
  • Humans
  • Kidney / metabolism*
  • Kidney Neoplasms / metabolism*
  • Male
  • Middle Aged
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Telomeric Repeat Binding Protein 1 / biosynthesis*
  • Telomeric Repeat Binding Protein 1 / genetics

Substances

  • RNA, Messenger
  • Telomeric Repeat Binding Protein 1