This report presents dietary intake estimates for fats and fatty acids from the National Health and Nutrition Examination Survey, 1999-2000, for the U.S. population. These include: total fat; total saturated, monounsaturated, and polyunsaturated fat; individual fatty acids; and cholesterol. Fat intakes are estimated from one 24-hour dietary recall interview. Population means, medians, and standard errors of the mean are weighted to produce national estimates and are presented by sex and age groups. Assessment of dietary intakes is an important part of monitoring the nutritional status of the U.S. population.