Plant transformation: a simple particle bombardment device based on flowing helium

Plant Mol Biol. 1992 Feb;18(4):835-9. doi: 10.1007/BF00020031.

Abstract

We observed that flowing helium at moderate pressures accelerated DNA-coated microprojectiles to velocities suitable for penetration of cells in intact plant tissues. The flowing helium principle permitted the construction of a simple and inexpensive transformation device that was easier to use than those previously described. This device provided efficient transformation of cells in soybean seedlings and other plants.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Equipment and Supplies
  • Genetic Techniques
  • Glycine max / genetics*
  • Helium*
  • Transformation, Genetic*

Substances

  • Helium