Beneficial effect of hyperbaric oxygen therapy on liver regeneration after 90% hepatectomy in rats

Eur Surg Res. Nov-Dec 2004;36(6):350-6. doi: 10.1159/000081643.

Abstract

Background/aims: Hyperbaric oxygen therapy (HBOT) has been reported to augment oxygen delivery to ischemic tissues and improve the liver dysfunction in clinical cases. HBOT was performed after 90% hepatectomy in rats to determine its effect on the regeneration of remnant liver.

Methods: After 90% hepatectomy was performed in 8-week-old male Wistar rats, the animals were subdivided into an HBOT (2 atm abs., 80% O2, 1 h/day, 3 days) group and a non-HBOT group. Members of both groups were sacrificed, usually every 4 h until a maximum of 50 h after hepatectomy, and the liver regeneration rate, the proportion of PCNA-positive cells and the ATP volume in the remnant tissues were examined.

Results: In the HBOT group, the liver regeneration rate at 36 h and 50 h after operation and the proportion of PCNA positive cells at 8 h was significantly increased compared with the non-HBOT group. The ATP volume in the remnant livers in the HBOT group was also significantly increased at 12 h.

Conclusion: HBOT augmented liver regeneration after hepatectomy by stabilization of energy metabolism induced by oxygen delivery in rats.

MeSH terms

  • Animals
  • Energy Metabolism / physiology
  • Hepatectomy*
  • Hyperbaric Oxygenation*
  • Liver Regeneration / physiology*
  • Male
  • Models, Animal
  • Oxygen / metabolism
  • Rats
  • Rats, Wistar

Substances

  • Oxygen