Reduction of oxidative stress marker in lung fluid of preterm infants after administration of intra-tracheal liposomal glutathione

Biol Neonate. 2005;87(3):178-80. doi: 10.1159/000082623. Epub 2004 Dec 9.

Abstract

Background: Low levels of glutathione are associated with subsequent chronic lung disease in preterm infants. Incorporation of glutathione into liposomes offers a method of increasing levels with a prolonged half-life compared with direct inhalation.

Objectives: The aim of this study was to examine the clinical feasibility of administering a single dose of liposomal glutathione and its effectiveness at raising glutathione at 12 and 24 h after treatment.

Methods: Fourteen ventilated preterm infants from the Regional Neonatal Intensive Care Unit at Liverpool Women's Hospital received 1 mg/kg or 10 mg/kg liposomal glutathione intra-tracheally and bronchoalveolar lavage fluid was collected prior to treatment, 12 and 24 h after dosing for glutathione and malondialdehyde estimation.

Results: Mean glutathione was initially 12.2 micromol/l, increasing to 52.8 micromol/l at 12 h (p = 0.006). Mean malondialdehyde was initially 265.6 nmol/l decreasing to 11.2 nmol/l at 12 h (p = 0.018).

Conclusions: Intra-tracheal liposomal glutathione instillation offers a feasible method of raising pulmonary glutathione in preterm infants and shows biochemical antioxidant effects.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers / metabolism*
  • Bronchoalveolar Lavage Fluid / chemistry*
  • Dose-Response Relationship, Drug
  • Feasibility Studies
  • Glutathione / administration & dosage*
  • Glutathione / metabolism
  • Glutathione / therapeutic use
  • Humans
  • Infant, Newborn
  • Infant, Premature*
  • Intubation, Intratracheal
  • Liposomes
  • Lung / metabolism*
  • Malondialdehyde / metabolism
  • Oxidation-Reduction
  • Oxidative Stress / drug effects*
  • Respiration, Artificial
  • Respiratory Distress Syndrome, Newborn / drug therapy*
  • Respiratory Distress Syndrome, Newborn / metabolism
  • Respiratory Distress Syndrome, Newborn / therapy
  • Time Factors

Substances

  • Biomarkers
  • Liposomes
  • Malondialdehyde
  • Glutathione