Extended Virulence Genotypes and Phylogenetic Background of Escherichia Coli Isolates From Patients With Cystitis, Pyelonephritis, or Prostatitis

J Infect Dis. 2005 Jan 1;191(1):46-50. doi: 10.1086/426450. Epub 2004 Nov 30.

Abstract

Molecular analysis of 63 Escherichia coli urine isolates showed that pyelonephritis (n=23) and prostatitis (n=17) isolates exhibited more virulence factors (VFs) among the 35 sought than did cystitis isolates (n=23). Several nontraditional VFs--including bmaE (M fimbriae), gafD (G fimbriae), fyuA (yersiniabactin receptor), ireA and iroN (novel siderophore receptors), cvaC (colicin [microcin] V), traT (serum-resistance associated), ibeA (invasion of brain endothelium), ompT (outer membrane protease T), and malX (pathogenicity island marker)--either differentiated significantly between syndromes (despite small numbers of isolates and possible multiple-comparison artifacts) or were broadly prevalent. Thus, interventions that target conserved uro-VFs may be possible, despite the likely existence of syndrome-specific pathogenetic mechanisms and/or host defense systems.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adhesins, Bacterial / genetics
  • Adult
  • Aged
  • Bacterial Outer Membrane Proteins / genetics
  • Bacteriocins / genetics
  • Cystitis / microbiology*
  • Escherichia coli / classification
  • Escherichia coli / genetics*
  • Escherichia coli / isolation & purification
  • Escherichia coli / pathogenicity
  • Escherichia coli Infections / microbiology*
  • Escherichia coli Proteins / genetics
  • Female
  • Fimbriae Proteins / genetics
  • Fimbriae, Bacterial / genetics
  • Genomic Islands / genetics
  • Humans
  • Lectins / genetics
  • Male
  • Membrane Proteins / genetics
  • Middle Aged
  • Peptide Hydrolases
  • Phenols / analysis
  • Porins / genetics
  • Prostatitis / microbiology*
  • Pyelonephritis / microbiology*
  • Receptors, Cell Surface / genetics
  • Spain
  • Thiazoles / analysis
  • Urine / microbiology
  • Virulence Factors / analysis
  • Virulence Factors / genetics*

Substances

  • Adhesins, Bacterial
  • Bacterial Outer Membrane Proteins
  • Bacteriocins
  • CusC protein, E coli
  • Escherichia coli Proteins
  • GafD protein, E coli
  • IreA protein, E coli
  • Lectins
  • Membrane Proteins
  • Phenols
  • Porins
  • Receptors, Cell Surface
  • Thiazoles
  • TraT protein, E coli
  • Virulence Factors
  • ompT protein, E coli
  • siderophore receptors
  • yersiniabactin
  • microcin
  • Fimbriae Proteins
  • Peptide Hydrolases