Effects of beta-Aescin on the expression of nuclear factor-kappaB and tumor necrosis factor-alpha after traumatic brain injury in rats

J Zhejiang Univ Sci B. 2005 Jan;6(1):28-32. doi: 10.1631/jzus.2005.B0028.


To investigate the inhibiting effect of beta-Aescin on nuclear factor-kappaB (NF-kappaB) activation and the expression of tumor necrosis factor-alpha (TNF-alpha) protein after traumatic brain injury (TBI) in the rat brain, 62 SD rats were subjected to lateral cortical impact injury caused by a free-falling object and divided randomly into four groups: (1) sham operated (Group A); (2) injured (Group B); (3) beta-Aescin treatment (Group C); (4) pyrrolidine dithocarbamate (PDTC) treatment (Group D). Beta-Aescin was administered in Group C and PDTC treated in Group D immediately after injury. A series of brain samples were obtained directly 6 h, 24 h and 3 d respectively after trauma in four groups. NF-kappaB activation was examined by Electrophoretic Mobility Shift Assay (EMSA); the levels of TNF-alpha protein were measured by radio-immunoassay (RIA); the water content of rat brain was measured and pathomorphological observation was carried out. NF-kappaB activation, the levels of TNF-alpha protein and the water content of rat brain were significantly increased (P<0.01) following TBI in rats. Compared with Group B, NF-kappaB activation (P<0.01), the levels of TNF-alpha protein (P<0.01) and the water content of brain (P<0.05) began to decrease obviously after injury in Groups C and D. Beta-Aescin could dramatically inhibit NF-kappaB activation and the expression of TNF-alpha protein in the rat brain, alleviate rat brain edema, and that could partially be the molecular mechanism by which beta-Aescin attenuates traumatic brain edema.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Body Water / metabolism*
  • Brain Edema / etiology
  • Brain Edema / metabolism
  • Brain Edema / pathology
  • Brain Edema / prevention & control
  • Brain Injuries / complications
  • Brain Injuries / drug therapy
  • Brain Injuries / metabolism*
  • Brain Injuries / pathology*
  • Escin / administration & dosage*
  • NF-kappa B / antagonists & inhibitors
  • NF-kappa B / metabolism*
  • Pyrrolidines / administration & dosage*
  • Rats
  • Rats, Sprague-Dawley
  • Thiocarbamates / administration & dosage*
  • Treatment Outcome
  • Tumor Necrosis Factor-alpha / metabolism*


  • NF-kappa B
  • Pyrrolidines
  • Thiocarbamates
  • Tumor Necrosis Factor-alpha
  • pyrrolidine dithiocarbamic acid
  • Escin