New constituents from Crinum latifolium with inhibitory effects against tube-like formation of human umbilical venous endothelial cells

Nat Prod Res. 2004 Dec;18(6):485-91. doi: 10.1080/1057563031000122103.

Abstract

Six compounds (1-6) were isolated from the methanol extract of Crinum latifolium by bioassay-guided separation. Among the six isolates, compounds 2 and 6 were new metabolites. Their structures were established as 4-senecioyloxymethyl-3,4-dimethoxycoumarin (2) and 5,6,3'-trihydroxy-7,8,4'-trimethoxyflavone (6) based on spectroscopic analyses. Compound 2 was found to be strongly inhibitory against the in vitro tube-like formation of human umbilical venous endothelial cells (HUVECs) while manifesting no cytotoxicity in tumor cell lines (B16F10, HCT116). Significant inhibitory activity (inhibition percentage, 53.5%) was still observed at concentrations as low as 1 microg/mL. Compound 6 showed a modest inhibitory effect on the tube-like formation of HUVECs. Other compounds, including cycloartenol (1), 4',7-dihydroxy-3'-methoxyflavan (3), 4',7-dihydroxyflavan (4), and 2',4',7-trihydroxydihydrochalcone (5) were found to be nearly inactive.

MeSH terms

  • Angiogenesis Inhibitors / administration & dosage
  • Angiogenesis Inhibitors / pharmacology*
  • Angiogenesis Inhibitors / therapeutic use
  • Animals
  • Antineoplastic Agents, Phytogenic / administration & dosage
  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Antineoplastic Agents, Phytogenic / therapeutic use
  • Cell Line, Tumor / drug effects
  • Coumarins / administration & dosage
  • Coumarins / pharmacology
  • Coumarins / therapeutic use
  • Crinum*
  • Endothelium, Vascular / drug effects
  • Flavonoids / administration & dosage
  • Flavonoids / pharmacology
  • Flavonoids / therapeutic use
  • Humans
  • Mice
  • Phytotherapy*
  • Plant Extracts / administration & dosage
  • Plant Extracts / pharmacology*
  • Plant Extracts / therapeutic use
  • Plant Leaves
  • Umbilicus

Substances

  • Angiogenesis Inhibitors
  • Antineoplastic Agents, Phytogenic
  • Coumarins
  • Flavonoids
  • Plant Extracts