Fetal-type phosphorylation of the tau in paired helical filaments

J Neurochem. 1992 May;58(5):1667-75. doi: 10.1111/j.1471-4159.1992.tb10039.x.


To determine the phosphorylation sites of the tau in paired helical filaments (PHF), two types of PHF antisera with different specificities were used: One was a conventional anti-PHF, and the other was an antiserum to formic acid-denatured PHF (anti-HFoPHF). Phosphorylated tau-specific antibodies, anti-ptau 1 and anti-ptau 2, were prepared from anti-PHF and anti-HFoPHF, respectively. We found that both anti-ptau 1 and anti-ptau 2 labeled fetal or juvenile tau but not adult tau. The anti-ptau 1- and anti-ptau 2-recognition sites were immunochemically localized to the fragment Asp313 to Ile328 in the most COOH-terminal portion of tau. Furthermore, Ser315 was determined as the anti-ptau 2 recognition site. The sequence surrounding Ser315 was not found in the canonical sequences phosphorylated with known kinases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies / immunology
  • Enzyme-Linked Immunosorbent Assay
  • Fetus / metabolism*
  • Humans
  • Immune Sera / immunology
  • Molecular Sequence Data
  • Neurofibrillary Tangles / immunology
  • Neurofibrillary Tangles / metabolism*
  • Neurofibrillary Tangles / ultrastructure
  • Phosphorylation
  • Rats
  • tau Proteins / chemistry
  • tau Proteins / metabolism*


  • Antibodies
  • Immune Sera
  • tau Proteins