Modulation of flowering responses in different Nicotiana varieties

Plant Mol Biol. 2004 May;55(2):253-62. doi: 10.1007/s11103-004-0557-8.

Abstract

We have identified and characterized a FLOWERING PROMOTING FACTOR 1 ( FPF1 ) gene from tobacco ( NtFPF1 ). Over-expression of NtFPF1 leads to early flowering in the day-neutral tobacco Nicotiana tabacum cv. Hicks, and under inductive photoperiods also in the short-day Nicotiana tabacum cv. Hicks Maryland Mammoth ( MM ) tobacco and the long-day plant Nicotiana sylvestris . N. sylvestris wild-type plants remained in the rosette stage and never flowered under non-inductive short-days, whereas 35S:: NtFPF1 transgenic plants bolted but did not flower. However, if treated with gibberellins, transgenic N. sylvestris plants flowered much faster under non-inductive short days than corresponding wild type plants, indicating an additive effect of gibberellins and the NtFPF1 protein in flowering time control. The day-neutral wild type cv. Hicks and the short-day cv. Hicks MM plants exhibit an initial rosette stage, both under short- and long-days. In the transgenic lines, this rosette stage was completely abolished. Wild-type plants of cv. Hicks MM never flowered under long days; however, all transgenic lines over-expressing NtFPF1 flowered under this otherwise non-inductive photoperiod.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • DNA, Plant / chemistry
  • DNA, Plant / genetics
  • DNA, Plant / isolation & purification
  • Flowers / drug effects
  • Flowers / genetics*
  • Flowers / growth & development
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Plant
  • Gibberellins / pharmacology
  • Molecular Sequence Data
  • Plant Proteins / genetics*
  • Plants, Genetically Modified
  • RNA, Plant / genetics
  • RNA, Plant / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Species Specificity
  • Tobacco / drug effects
  • Tobacco / genetics*
  • Tobacco / growth & development

Substances

  • DNA, Plant
  • Gibberellins
  • Plant Proteins
  • RNA, Plant

Associated data

  • GENBANK/AY496934