Study on the interactions between anti-HIV-1 active compounds with trans-activation response RNA by affinity capillary electrophoresis

Li Ding; Xin-Xiang Zhang; Wen-Bao Chang; Wei Lin; Ming Yang

doi:10.1016/j.jchromb.2004.10.002

Study on the interactions between anti-HIV-1 active compounds with trans-activation response RNA by affinity capillary electrophoresis

J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Jan 5;814(1):99-104. doi: 10.1016/j.jchromb.2004.10.002.

Authors

Li Ding¹, Xin-Xiang Zhang, Wen-Bao Chang, Wei Lin, Ming Yang

Affiliation

¹ College of Chemistry, Peking University, Beijing 100871, China.

PMID: 15607713
DOI: 10.1016/j.jchromb.2004.10.002

Abstract

The study on the interactions between two anti-human immunodeficiency virus type 1 (anti-HIV-1) active compounds with trans-activation response (TAR) RNA by affinity capillary electrophoresis (ACE) with UV absorbance detection is presented. The results showed that the novel active molecules could interact with TAR RNA and inhibit the reproduce process of HIV-1. The binding constants were estimated by the change of migration time of the analytes through the change of concentrations of TAR RNA in the buffer solution. The yielded binding constants of 8.87 x 10(3)M(-1) for active compound C(3) and 8.42 x 10(3)M(-1) for MC(3) at 20.0 degrees C, 0.626 x 10(3)M(-1) and 0.644 x 10(3)M(-1) at 37.0 degrees C, respectively. The thermodynamic parameters Delta H and DeltaS were obtained and shown that both hydrophobic and electrostatic interaction played roles in the binding processes. The results showed that the presented method was an easy and simple method to evaluate the interaction of small molecules with some bioactive materials.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-HIV Agents / pharmacology*
Electrophoresis, Capillary / methods*
RNA / drug effects*
Spectrophotometry, Ultraviolet
Thermodynamics

Substances

Anti-HIV Agents
RNA