A novel potassium channel encoded by Ectocarpus siliculosus virus

Biochem Biophys Res Commun. 2005 Jan 28;326(4):887-93. doi: 10.1016/j.bbrc.2004.11.125.


Kcv, the first identified viral potassium channel encoded by the green algae Paramecium bursaria chlorella virus (PBCV-1), conducted K(+) selective currents when expressed in heterologous systems. This K(+) channel was proposed to be important for PBCV-1 infection and replication. In the present study, we identified and functionally characterized a novel K(+) channel Kesv, encoded by Ectocarpus siliculosus virus that infects filamentous marine brown algae. Kesv encodes a protein of 124 amino acids and is 21.8% identical and 37.1% homologous to Kcv. Membrane topology programs predicted that Kesv consists of three transmembrane domains. When expressed in Xenopus oocytes, Kesv induced largely instantaneous, K(+) selective currents that were sensitive to block by Ba(2+) and amantadine. Thus, Kesv along with Kcv, constitutes an emerging family of viral potassium channels, which may play important roles in the life cycle of viruses.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Cells, Cultured
  • Gene Expression Regulation, Viral / physiology
  • Membrane Potentials / physiology
  • Oocytes / physiology*
  • Phycodnaviridae / classification
  • Phycodnaviridae / genetics*
  • Phycodnaviridae / metabolism*
  • Potassium / metabolism*
  • Potassium Channels / chemistry*
  • Potassium Channels / classification
  • Potassium Channels / physiology*
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism
  • Species Specificity
  • Structure-Activity Relationship
  • Viral Proteins / chemistry
  • Viral Proteins / metabolism
  • Xenopus laevis


  • Potassium Channels
  • Recombinant Proteins
  • Viral Proteins
  • Potassium