Rapid construction of capsid-modified adenoviral vectors through bacteriophage lambda Red recombination

Hum Gene Ther. 2004 Nov;15(11):1125-30. doi: 10.1089/hum.2004.15.1125.


There are extensive efforts to develop cell-targeting adenoviral vectors for gene therapy wherein endogenous cell-binding ligands are ablated and exogenous ligands are introduced by genetic means. Although current approaches can genetically manipulate the capsid genes of adenoviral vectors, these approaches can be time-consuming and require multiple steps to produce a modified viral genome. We present here the use of the bacteriophage lambda Red recombination system as a valuable tool for the easy and rapid construction of capsid-modified adenoviral genomes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenoviridae / genetics*
  • Bacteriophage lambda / genetics*
  • Biotin / genetics
  • Biotinylation
  • Blotting, Western
  • Capsid*
  • Cell Line
  • Cloning, Molecular
  • DNA / metabolism
  • Enzyme-Linked Immunosorbent Assay
  • Genetic Engineering
  • Genetic Therapy
  • Genetic Vectors*
  • Genome, Viral
  • Humans
  • Ligands
  • Models, Genetic
  • Peptide Library
  • Peptides / chemistry
  • Plasmids / metabolism
  • Protein Structure, Tertiary
  • Recombination, Genetic*
  • Time Factors


  • Ligands
  • Peptide Library
  • Peptides
  • Biotin
  • DNA