From worm to human: bioinformatics approaches to identify FOXO target genes

Mech Ageing Dev. 2005 Jan;126(1):209-15. doi: 10.1016/j.mad.2004.09.021.


Longevity regulatory genes include the Forkhead transcription factor FOXO, in addition to NAD-dependent histone deacetylase silent information regulator 2 (Sir2). The FOXO/DAF-16 family of transcription factors constitute an evolutionarily conserved subgroup within a larger family known as winged helix or Forkhead transcriptional regulators. Here we demonstrate how to identify FOXO target genes and their potential cis-regulatory binding sites in the promoters via bioinformatics approaches. These results provide new testable hypotheses for further experimental verifications.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Computational Biology / methods
  • Forkhead Transcription Factors
  • Gene Expression Regulation / genetics*
  • Gene Expression Regulation / physiology
  • Humans
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / metabolism
  • Response Elements / genetics*
  • Sequence Analysis, DNA / methods*
  • Signal Transduction / genetics*
  • Signal Transduction / physiology
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism


  • Forkhead Transcription Factors
  • Nuclear Proteins
  • Transcription Factors