Genomic and cytological analysis of the Y chromosome of Drosophila melanogaster: telomere-derived sequences at internal regions

Chromosoma. 2004 Dec;113(6):295-304. doi: 10.1007/s00412-004-0318-0. Epub 2004 Nov 3.

Abstract

The genomic analysis of heterochromatin is essential for studying chromosome behavior as well as for understanding chromosome evolution. The Y chromosome of Drosophila melanogaster is entirely heterochromatic and the under-representation of this chromosome in genomic libraries together with the difficulty of assembling its sequence has made its study very difficult. Here, we present the construction of bacterial artificial chromosome (BAC) contigs from regions h14, h16 and the centromeric region h18. The analysis of these contigs shows that telomere-derived sequences are present at internal regions. In addition, immunostaining of prometaphase chromosomes with an antibody to the kinetochore-specific protein BubR1 has revealed the presence of this protein in some Y chromosome regions rich in telomere-related sequences. Collectively, our data provide further evidence for the hypothesis that the Drosophila Y chromosomes might have evolved from supernumerary chromosomes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / immunology
  • Base Sequence
  • Cell Cycle Proteins
  • Chromosomes, Artificial, Bacterial / genetics
  • Chromosomes, Artificial, Yeast / genetics
  • Contig Mapping
  • DNA Transposable Elements
  • Drosophila Proteins / genetics
  • Drosophila Proteins / metabolism
  • Drosophila Proteins / physiology
  • Drosophila melanogaster / genetics*
  • Gene Products, gag / genetics
  • Gene Products, gag / metabolism
  • Gene Products, gag / physiology
  • Genomics
  • Heterochromatin / genetics*
  • In Situ Hybridization, Fluorescence
  • Male
  • Molecular Sequence Data
  • Protein Kinases / analysis
  • Protein Kinases / immunology
  • Protein Kinases / metabolism
  • Protein-Serine-Threonine Kinases
  • Telomere / genetics*
  • Y Chromosome / chemistry
  • Y Chromosome / genetics*
  • Y Chromosome / metabolism

Substances

  • Antibodies
  • Cell Cycle Proteins
  • DNA Transposable Elements
  • Drosophila Proteins
  • Gene Products, gag
  • HeT-A-gag protein, Drosophila
  • Heterochromatin
  • TART-element-gag protein, Drosophila
  • Protein Kinases
  • Bub1 spindle checkpoint protein
  • Protein-Serine-Threonine Kinases

Associated data

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