Overexpression of a family of RPEL proteins modifies cell shape

FEBS Lett. 2005 Jan 3;579(1):100-4. doi: 10.1016/j.febslet.2004.11.054.

Abstract

Proteins containing RPEL motifs (e.g., MAL) are important in the regulation of gene expression by the actin cytoskeleton. Screening the ENSEMBL database for RPEL proteins identified four additional proteins that contain RPEL motifs and nuclear localisation sequences, three of which (RPEL-A, RPEL-B and RPEL-C) are expressed in adult mouse tissues with different expression profiles. The mRNAs encoding RPEL-B and RPEL-C were subject to alternative splicing. Expression of these genes in cells indicated that they had a marked effect on cell shape. Furthermore, when expressed with a nuclear localised actin all of the different forms became restricted to the nucleus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / analysis
  • Actins / metabolism*
  • Alternative Splicing
  • Amino Acid Motifs / genetics
  • Amino Acid Sequence
  • Animals
  • Cell Nucleus / chemistry
  • Cell Nucleus / metabolism
  • Cell Shape / genetics
  • Cells, Cultured
  • Computational Biology
  • Gene Expression
  • Gene Expression Regulation
  • Mice
  • Microfilament Proteins / genetics
  • Molecular Sequence Data
  • Muscle Proteins / genetics
  • Nuclear Localization Signals / genetics
  • Nuclear Localization Signals / metabolism
  • Nuclear Proteins / analysis
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • Promoter Regions, Genetic / genetics
  • Sequence Alignment
  • Tissue Distribution

Substances

  • Actins
  • Microfilament Proteins
  • Muscle Proteins
  • Nuclear Localization Signals
  • Nuclear Proteins
  • transgelin