Interplay of pu.1 and gata1 Determines Myelo-Erythroid Progenitor Cell Fate in Zebrafish

Dev Cell. 2005 Jan;8(1):97-108. doi: 10.1016/j.devcel.2004.11.014.

Abstract

The zebrafish is a powerful model system for investigating embryonic vertebrate hematopoiesis, allowing for the critical in vivo analysis of cell lineage determination. In this study, we identify zebrafish myeloerythroid progenitor cells (MPCs) that are likely to represent the functional equivalent of mammalian common myeloid progenitors. Utilizing transgenic pu.1-GFP fish, real-time MPC differentiation was correlated with dynamic changes in cell motility, morphology, and gene expression. Unlike mammalian hematopoiesis, embryonic zebrafish myelopoiesis and erythropoiesis occur in anatomically separate locations. Gene knockdown experiments and transplantation assays demonstrated the reciprocal negative regulation of pu.1 and gata1 and their non-cell-autonomous regulation that determines myeloid versus erythroid MPC fate in the distinct blood-forming regions. Furthermore, forced expression of pu.1 in the bloodless mutant cloche resulted in myelopoietic rescue, providing intriguing evidence that this gene can function in the absence of some stem cell genes, such as scl, in governing myelopoiesis.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Cell Differentiation / physiology
  • Cell Movement / physiology
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / physiology*
  • Embryonic Induction
  • Erythroid Precursor Cells / physiology*
  • Erythroid-Specific DNA-Binding Factors
  • Flow Cytometry / methods
  • GATA1 Transcription Factor
  • Gene Expression Regulation, Developmental / physiology
  • Genotype
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Hematopoiesis / physiology
  • In Situ Hybridization / methods
  • Microinjections / methods
  • Models, Biological
  • Myeloid Progenitor Cells / physiology*
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins / physiology*
  • RNA, Messenger / biosynthesis
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • Trans-Activators / genetics
  • Trans-Activators / physiology*
  • Transcription Factors / genetics
  • Transcription Factors / physiology*
  • Transplantation / methods
  • Zebrafish / embryology
  • Zebrafish Proteins / genetics
  • Zebrafish Proteins / metabolism

Substances

  • DNA-Binding Proteins
  • Erythroid-Specific DNA-Binding Factors
  • GATA1 Transcription Factor
  • Proto-Oncogene Proteins
  • RNA, Messenger
  • Trans-Activators
  • Transcription Factors
  • Zebrafish Proteins
  • gata1a protein, zebrafish
  • proto-oncogene protein Spi-1
  • Green Fluorescent Proteins