Monitoring of CD4+ and CD8+ T-cell responses after dendritic cell-based immunotherapy using CFSE dye dilution analysis

J Clin Immunol. 2004 Nov;24(6):653-63. doi: 10.1007/s10875-004-6237-2.


CFSE dye dilution analysis and [3H] thymidine incorporation were used side by side to assess proliferative responses of peripheral blood mononuclear cells (PBMCs) after vaccination of renal cell carcinoma patients (n=6) with antigen-loaded dendritic cells. Immune responses against the control antigen keyhole limpet hemocyanin (KLH) were induced in all patients. While [3H] thymidine incorporation revealed a 4 to 977-fold increase in KLH-induced proliferation (mean: 209-fold), CFSE-labeling experiments demonstrated that the KLH-responsive population of postvaccination PBMCs represented 7-53% (mean: 23%). Combining CFSE-labeling with T-cell subset analysis confirmed the presence of CD4+ KLH-reactive T cells but also revealed a substantial population of CD8+ KLH-reactive T cells in one patient as well as minor populations of CD8+ KLH-reactive T cells in three other patients. Our data indicate that CFSE dye dilution analysis is a valuable tool for immune monitoring after dendritic cell vaccination.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • CD4-Positive T-Lymphocytes / immunology*
  • CD8-Positive T-Lymphocytes / immunology*
  • Carcinoma, Renal Cell / therapy
  • Cell Proliferation
  • Coloring Agents*
  • Cytokines / analysis
  • Dendritic Cells / immunology
  • Dendritic Cells / transplantation*
  • Flow Cytometry / methods
  • Hemocyanins / immunology
  • Humans
  • Immunotherapy, Adoptive / methods*
  • Leukocytes, Mononuclear / cytology
  • Methods


  • Coloring Agents
  • Cytokines
  • Hemocyanins
  • keyhole-limpet hemocyanin