Particulate DNA polymerase from the cytoplasm of Xenopus laevis oocytes

Biochem Biophys Res Commun. 1976 Dec 20;73(4):985-92. doi: 10.1016/0006-291x(76)90219-9.


A DNA polymerase has been partially purified and characterized from Xenopus laevis stage 6 oocytes. The enzyme is present only in the cytoplasm and has been shown to be able to copy Poly(A) x oligo(dT), to be sensitive to N-ethylmaleimide, and to sediment faster than 4 S in high salt glycerol gradient. The enzyme can be extracted from particulate material which has a density in sucrose gradient ranging from 1.200 to 1.225 g/cc. This particulate material is identified by its ability to use Poly(A) x oligo(dT) as template in an exogenous DNA polymerase reaction and by its endogenous DNA synthesizing capacity.

MeSH terms

  • Animals
  • Centrifugation, Density Gradient
  • Chromatography, DEAE-Cellulose
  • Cytoplasm / enzymology*
  • DNA / biosynthesis
  • DNA-Directed DNA Polymerase / chemistry
  • DNA-Directed DNA Polymerase / isolation & purification*
  • DNA-Directed DNA Polymerase / metabolism*
  • Ethylmaleimide / pharmacology
  • Nucleic Acid Synthesis Inhibitors
  • Oocytes / enzymology*
  • Potassium Chloride / pharmacology
  • Xenopus laevis*


  • Nucleic Acid Synthesis Inhibitors
  • Potassium Chloride
  • DNA
  • DNA-Directed DNA Polymerase
  • Ethylmaleimide