Phase 1 study of multiple biomarkers for metabolism and oxidative stress after one-week intake of broccoli sprouts

Biofactors. 2004;22(1-4):271-5. doi: 10.1002/biof.5520220154.


Little is known about the direct effect of broccoli sprouts on human health. So we investigated the effect of broccoli sprouts on the induction of various biochemical oxidative stress markers. Twelve healthy subjects (6 males and 6 females) consumed fresh broccoli sprouts (100 g/day) for 1 week for a phase 1 study. Before and after the treatment, biochemical examination was conducted and natural killer cell activity, plasma amino acids, plasma PCOOH (phosphatidylcholine hydroperoxide), the serum coenzyme Q(10), urinary 8-isoprostane, and urinary 8-OHdG (8-hydroxydeoxyguanosine) were measured. With treatment, total cholesterol and LDL cholesterol decreased, and HDL cholesterol increased significantly. Plasma cystine decreased significantly. All subjects showed reduced PCOOH, 8-isoprostane and 8-OHdG, and increased CoQ(10)H(2)/CoQ(10) ratio. Only one week intake of broccoli sprouts improved cholesterol metabolism and decreased oxidative stress markers.

Publication types

  • Clinical Trial
  • Clinical Trial, Phase I
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / blood
  • Biomarkers / blood*
  • Blood Urea Nitrogen
  • Brassica*
  • Coenzymes
  • Female
  • Humans
  • Killer Cells, Natural / immunology
  • Lipids / blood
  • Liver Function Tests
  • Male
  • Oxidative Stress*
  • Phosphatidylcholines / blood
  • Reference Values
  • Ubiquinone / analogs & derivatives*
  • Ubiquinone / blood


  • Amino Acids
  • Biomarkers
  • Coenzymes
  • Lipids
  • Phosphatidylcholines
  • phosphatidylcholine hydroperoxide
  • Ubiquinone
  • coenzyme Q10