In vitro and in vivo evaluation of antibiotic diffusion from antibiotic-impregnated polymethylmethacrylate beads

Clin Orthop Relat Res. 1992 May:(278):244-52.

Abstract

The elution of antibiotics from antibiotic-impregnated polymethylmethacrylate (PMMA) beads was measured in mongrel dogs. The antibiotics, used in mixture with Simplex cement, included cefazolin (Ancef; 4.5 g/40 g cement powder), ciprofloxacin (Cipro; 6 g/40 g powder), clindamycin (Cleocin; 6 g/40 g powder), ticarcillin (Ticar; 12 g/40 g powder), tobramycin (Nebcin; 9.8 g/40 g powder), and vancomycin (Vancocin; 4 g/40 g powder). After a pneumatic drill was used to dredge a trough in the tibia, five beads were implanted. During the next 28 days, seroma samples and serum samples were taken for antibiotic measurements. On Day 28, the dogs were killed, beads removed, and the seroma, serum, bone, and granulation tissue sampled. The results of the study showed that clindamycin, vancomycin, and tobramycin exhibited good elution characteristics and had consistently high levels in bone and granulation tissue.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Bacterial Agents / administration & dosage
  • Anti-Bacterial Agents / metabolism*
  • Bone and Bones / chemistry
  • Cefazolin / metabolism
  • Ciprofloxacin / metabolism
  • Clindamycin / metabolism
  • Diffusion
  • Dogs
  • Granulation Tissue / chemistry
  • Methylmethacrylates / metabolism*
  • Ticarcillin / metabolism
  • Tobramycin / metabolism
  • Vancomycin / metabolism

Substances

  • Anti-Bacterial Agents
  • Methylmethacrylates
  • Clindamycin
  • Ciprofloxacin
  • Vancomycin
  • Ticarcillin
  • Cefazolin
  • Tobramycin