Cloning and sequence analysis of a rapamycin-binding protein-encoding gene (RBP1) from Candida albicans

Gene. 1992 Apr 1;113(1):125-7. doi: 10.1016/0378-1119(92)90679-j.


Rapamycin (Rm) and FK506 are macrolide antifungal agents that exhibit potent immunosuppressive properties in higher eukaryotes which are mediated through interaction with specific receptor proteins (FKBPs or RBPs, for FK506- and Rm-binding proteins, respectively). These proteins possess peptidyl-prolyl cis-trans isomerase (PPIase) activity in vitro which is inhibited by the binding of Rm and FK506. We previously isolated a gene encoding an RBP from Saccharomyces cerevisiae, and demonstrated that null mutations in this gene (called RBP1) result in a recessive Rm-resistant (RmR) phenotype. We now have cloned the Candida albicans RBP1 gene via complementation of the RmR phenotype in S. cerevisiae. The predicted C. albicans RBP exhibits 61%, 52% and 49% amino acid (aa) sequence identity with RBPs (FKBPs) from S. cerevisiae, Neurospora crassa and human cells (FKBP-12), respectively. Furthermore, several of the aa residues identified as being important for drug binding in human FKBP-12 are conserved within the C. albicans RBP.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Antifungal Agents / metabolism*
  • Base Sequence
  • Candida albicans / genetics*
  • Candida albicans / metabolism
  • Carrier Proteins / genetics*
  • Cloning, Molecular / methods
  • Fungal Proteins / genetics*
  • Gene Library
  • Genes, Fungal*
  • Genetic Complementation Test
  • Heat-Shock Proteins / genetics*
  • Molecular Sequence Data
  • Polyenes / metabolism*
  • Restriction Mapping
  • Saccharomyces cerevisiae / genetics
  • Sequence Homology, Nucleic Acid
  • Sirolimus
  • Tacrolimus Binding Proteins


  • Antifungal Agents
  • Carrier Proteins
  • Fungal Proteins
  • Heat-Shock Proteins
  • Polyenes
  • Tacrolimus Binding Proteins
  • Sirolimus

Associated data

  • GENBANK/M76680
  • GENBANK/M76681
  • GENBANK/M76682
  • GENBANK/M79307
  • GENBANK/M79308
  • GENBANK/M79309
  • GENBANK/M79310
  • GENBANK/M84759
  • GENBANK/X60732
  • GENBANK/X60733