Nonlymphoid peritoneal cells suppress the T cell response to Mls

Immunobiology. 2004;209(8):575-84. doi: 10.1016/j.imbio.2004.07.002.

Abstract

Comparative analyses of the ability of lymphoid tissue to present the minor lymphocyte stimulatory (Mls) superantigen Mls-1a in vitro revealed that all tissues containing mature B cells, except peritoneal cavity (PerC) cells, induced Mls-1a-specific T cell activation. Irradiation and mitomycin C treatment, addition of IL-2 and IL-12, and neutralization of IL-10 and TGF-beta did not restore Mls-1a antigen presentation by PerC cells. Co-culture studies revealed that PerC cells actively suppress the T cell response to Mls-1a. PerC cells from severe-combined immune-defective (SCID) mice also suppressed this response indicating that nonlymphoid cells mediate this effect. These results suggest that in addition to antigen processing and presentation, resident peritoneal cavity cells may temper lymphocyte activation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Antigen-Presenting Cells / drug effects
  • Antigen-Presenting Cells / immunology*
  • Antigen-Presenting Cells / radiation effects
  • Apoptosis
  • B-Lymphocytes / immunology
  • Cell Communication / immunology
  • Coculture Techniques
  • Cytokines / immunology
  • Cytokines / pharmacology
  • Cytokines / physiology
  • Female
  • Immune Tolerance*
  • Lymphocyte Activation / immunology*
  • Lymphoid Tissue / cytology
  • Lymphoid Tissue / immunology
  • Male
  • Mice
  • Mice, Inbred Strains
  • Mice, Mutant Strains
  • Minor Lymphocyte Stimulatory Antigens / immunology*
  • Mitomycin / pharmacology
  • Peritoneal Cavity / cytology*
  • T-Lymphocytes / immunology*

Substances

  • Antibodies, Monoclonal
  • Cytokines
  • Minor Lymphocyte Stimulatory Antigens
  • Mitomycin