Polyunsaturated fatty acids sensitize human colon adenocarcinoma HT-29 cells to death receptor-mediated apoptosis

Cancer Lett. 2005 Jan 31;218(1):33-41. doi: 10.1016/j.canlet.2004.07.038.

Abstract

The proliferative and apoptotic response to TNF-alpha and anti-Fas antibody (CH-11) in human colon adenocarcinoma HT-29 cells was modulated by pretreatment with arachidonic (AA, 20:4, n-6) or docosahexaenoic (DHA, 22:6, n-3) fatty acids, which alone increased reactive oxygen species production and lipid peroxidation, and decreased the S-phase of the cell cycle. The higher amount of floating cells, subG0/G1 population and apoptotic cells detected in pre-treated cells was potentiated by cycloheximide. The effects of CH-11 were associated with activation of caspase-8, -9, and -3, cleavage of poly(ADP-ribose)polymerase-PARP, and decreased mitochondrial membrane potential (MMP), but these parameters were not significantly changed after PUFA pretreatment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / pathology*
  • Antibodies / pharmacology
  • Apoptosis / drug effects*
  • Arachidonic Acids / pharmacology*
  • Cell Proliferation
  • Collagen Type XI / metabolism
  • Colonic Neoplasms / pathology*
  • Docosahexaenoic Acids / pharmacology*
  • Humans
  • Lipid Peroxidation
  • Mitochondria
  • Poly (ADP-Ribose) Polymerase-1
  • Poly(ADP-ribose) Polymerases
  • Reactive Oxygen Species
  • Tumor Necrosis Factor-alpha / pharmacology

Substances

  • Antibodies
  • Arachidonic Acids
  • CH-11 anti-fas antibody, human
  • COL11A2 protein, human
  • Collagen Type XI
  • Reactive Oxygen Species
  • Tumor Necrosis Factor-alpha
  • Docosahexaenoic Acids
  • PARP1 protein, human
  • Poly (ADP-Ribose) Polymerase-1
  • Poly(ADP-ribose) Polymerases