Transcriptional analysis of genes encoding Shiga toxin 2 and its variants in Escherichia coli

Appl Environ Microbiol. 2005 Jan;71(1):558-61. doi: 10.1128/AEM.71.1.558-561.2005.

Abstract

Six of 37 non-O157 Escherichia coli strains possessing Shiga toxin (Stx) 2 gene variant stx(2d) or stx(2e) secreted no detectable Stx. These isolates produced significantly less stx mRNA than Stx2d, Stx2e, Stx2c, or Stx2 secretors did. Standard screening procedures miss a significant subset of E. coli harboring stx(2) variants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Chlorocebus aethiops
  • Escherichia coli / genetics
  • Escherichia coli / growth & development
  • Escherichia coli / metabolism*
  • Genetic Variation*
  • Humans
  • Molecular Sequence Data
  • Sequence Analysis, DNA
  • Shiga Toxin 2 / genetics*
  • Shiga Toxin 2 / metabolism
  • Shiga Toxin 2 / toxicity*
  • Transcription, Genetic*
  • Vero Cells

Substances

  • Shiga Toxin 2

Associated data

  • GENBANK/AJ567995
  • GENBANK/AJ567996
  • GENBANK/AJ567997
  • GENBANK/AJ567998