Abstract
A new plasmid for the overexpression of His-tagged thermozymes in Thermus thermophilus was developed. With this plasmid, soluble and active histidine-tagged DNA polymerase from T. thermophilus was overproduced in larger amounts in the thermophile than in Escherichia coli. The protein purified from the thermophile was active in PCR.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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DNA-Directed DNA Polymerase / genetics
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DNA-Directed DNA Polymerase / metabolism*
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Escherichia coli / enzymology
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Escherichia coli / genetics
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Gene Expression Regulation, Bacterial
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Genetic Techniques
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Histidine
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Polymerase Chain Reaction
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Thermus thermophilus / enzymology*
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Thermus thermophilus / genetics
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Thermus thermophilus / growth & development
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Up-Regulation*
Substances
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Histidine
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Tth polymerase
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DNA-Directed DNA Polymerase