Abstract
NMR studies, UV-monitored melting experiments, and ab initio calculations show that 5-hydroxyuracil, produced by the oxidative de-amination of cytosines by reactive oxygen species, can form stable base-pairs with dA, dG, dC and dT residues in a DNA duplex, providing a basis for the in-vivo incorporation of 5-hydroxyuracil during DNA replication.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Pairing*
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DNA / chemistry*
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Magnetic Resonance Spectroscopy
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Nucleic Acid Conformation
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Nucleosides / chemistry*
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Oxidation-Reduction
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Uracil / analogs & derivatives*
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Uracil / chemistry
Substances
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Nucleosides
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5-hydroxyuracil
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Uracil
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DNA