The inactivation of phospholipase A2 by scalaradial: a biomimetic study by electrospray mass spectrometry

Rapid Commun Mass Spectrom. 2005;19(3):303-8. doi: 10.1002/rcm.1785.

Abstract

A biomimetic approach was employed to shed light on the nature of chemical reactions occurring in the covalent inactivation of phospholipase A(2) (PLA(2)) by scalaradial (1), a marine dialdehyde terpenoid endowed with potent anti-inflammatory activity. To this end, a detailed study of the reaction profile between the nitrogenous nucleophile isopropylamine and scalaradial was performed under biologically relevant conditions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Inflammatory Agents, Non-Steroidal / chemistry*
  • Chromatography, High Pressure Liquid
  • Homosteroids / chemistry*
  • Phospholipases A / chemistry*
  • Phospholipases A2
  • Propylamines / chemistry*
  • Sesterterpenes
  • Spectrometry, Mass, Electrospray Ionization / methods*
  • Terpenes / chemistry*

Substances

  • Anti-Inflammatory Agents, Non-Steroidal
  • Homosteroids
  • Propylamines
  • Sesterterpenes
  • Terpenes
  • scalaradial
  • Phospholipases A
  • Phospholipases A2
  • 2-propylamine