Long-term treatment effects of Pueraria mirifica phytoestrogens on parathyroid hormone and calcium levels in aged menopausal cynomolgus monkeys

J Reprod Dev. 2004 Dec;50(6):639-45. doi: 10.1262/jrd.50.639.


To determine the effect of Pueraria mirifica (PM) on serum parathyroid hormone (PTH) and calcium levels on aged menopausal monkeys (Macaca fascicularis), subjects were treated with 10, 100, or 1,000 mg/day of PM. Blood samples were collected every 5 days for 30, 90, and 60 days during pre-treatment, treatment, and post-treatment periods, respectively. Sera were assayed for PTH, estradiol, and calcium levels. PM-1,000 had the strongest effect on the decrease in PTH (0.001<P< or =0.05) and calcium levels (0.001<P< or =0.03) during the treatment period. PTH levels remained low for the first 15 days of the post-treatment period (0.01< or =P < or =0.05). PM-10 induced a significant decrease in PTH level on day 80 (P=0.02) during the treatment period and a significant decrease in calcium level on day 75 (P<0.01). There were no changes in serum PTH and calcium levels throughout the study period in the PM-100 group. Estradiol levels decreased significantly during the treatment period in all treatment groups. The results suggest that long-term treatment with 1,000 mg/day of PM decreases serum PTH and calcium levels in aged menopausal monkeys, indicating that PM ameliorates bone loss caused by estrogen deficiency.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium / blood
  • Calcium / metabolism*
  • Dose-Response Relationship, Drug
  • Estradiol / blood
  • Female
  • Macaca fascicularis
  • Menopause
  • Parathyroid Hormone / blood
  • Parathyroid Hormone / metabolism*
  • Phytoestrogens / metabolism*
  • Phytoestrogens / therapeutic use*
  • Plant Preparations / therapeutic use
  • Pueraria / metabolism*
  • Time Factors


  • Parathyroid Hormone
  • Phytoestrogens
  • Plant Preparations
  • Estradiol
  • Calcium