Collagen-like peptide exhibits a remarkable antiwrinkle effect on the skin when topically applied: in vivo study

Int J Tissue React. 2004;26(3-4):105-11.


In this double-blind clinical study, we evaluated the effect of our newly developed synthetic collagen-like hexapeptide on wrinkles. Twenty healthy women volunteers, aged 40 to 62 years old, participated in the study Volunteers applied either a gel formula containing 3% of the collagen-like peptide and 1% of a booster molecule that stimulates general cell metabolism with no specific effect on wrinkles, or a placebo gel, on the eye zone area twice a day for 4 weeks. Control visits were performed at the beginning and the end of the study. Skin wrinkles were evaluated clinically and by silicon replica analysis followed by statistical treatment using the matched-pairs Student's t-test. The results showed that application of the collagen-like peptide on the skin significantly reduced the total surface of wrinkles and this effect was observed in 75% of the replicas. Similarly, the decrease in number and average depth of wrinkles was also significant and was observed in 65% and 75% of the replicas, respectively. The effect of the collagen-like peptide on reducing the total and average length of wrinkles was also remarkable. This effect was statistically highly significant (p < 0.003) and was observed in 75% to 80% of the replicas. Moreover these results were supported by volunteer questionnaires and clinical observation. The results demonstrate that the collagen-like peptide acts deeply and intensely on wrinkles; these properties are of great interest in the field of antiaging skin care research.

Publication types

  • Clinical Trial

MeSH terms

  • Administration, Topical
  • Adult
  • Collagen / genetics*
  • Female
  • Humans
  • Middle Aged
  • Peptides / administration & dosage
  • Peptides / genetics
  • Peptides / pharmacology*
  • Skin / drug effects*
  • Skin Aging / drug effects*


  • Peptides
  • Collagen