IMPs, VIMs and SPMs: The Diversity of Metallo-Beta-Lactamases Produced by Carbapenem-Resistant Pseudomonas Aeruginosa in a Brazilian Hospital

Clin Microbiol Infect. 2005 Jan;11(1):73-6. doi: 10.1111/j.1469-0691.2004.01031.x.

Abstract

Pseudomonas aeruginosa isolates (n=183), collected from bacteraemic patients hospitalised in Sao Paulo Hospital (Brazil) during 2000-2001, were screened for susceptibility to antimicrobial agents. The polymyxins were the most active compounds (100% susceptibility), followed by amikacin and cefepime (59.0%), meropenem (57.4%), and imipenem and gentamicin (55.2%). Imipenem-resistant isolates were ribotyped and screened for production of metallo-beta-lactamases (MBLs) by PCR with primers for bla(IMP), bla(VIM) and bla(SPM). MBL production was detected in 36 isolates (19.7% of the entire collection; 43.9% of the imipenem-resistant isolates) and the MBLs included SPM-1-like (55.6%), VIM-2-like (30.6%) and IMP-1-like (8.3%) enzymes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Bacteremia / microbiology
  • Brazil
  • Carbapenems / pharmacology*
  • Hospitals, Teaching*
  • Humans
  • Imipenem / pharmacology
  • Microbial Sensitivity Tests
  • Polymerase Chain Reaction
  • Polymyxins / pharmacology
  • Pseudomonas Infections / microbiology
  • Pseudomonas aeruginosa / drug effects*
  • Pseudomonas aeruginosa / enzymology
  • Pseudomonas aeruginosa / genetics
  • beta-Lactam Resistance*
  • beta-Lactamases / classification
  • beta-Lactamases / genetics*
  • beta-Lactamases / metabolism

Substances

  • Anti-Bacterial Agents
  • Carbapenems
  • Polymyxins
  • Imipenem
  • VIM-2 beta-lactamase
  • beta-lactamase IMP-1
  • beta-lactamase SPM-1, Pseudomonas aeruginosa
  • beta-Lactamases