Dominant-positive and dominant-negative heat shock factors

Methods. 2005 Feb;35(2):199-207. doi: 10.1016/j.ymeth.2004.08.010. Epub 2004 Dec 19.


Heat and other acute or chronic stresses provoke multiple cellular reactions, including activation of the heat shock or stress protein response. To date, no compounds have become available that specifically activate, or block activation of, the stress protein response. At the transcriptional level the response is mediated by heat shock factors, ubiquitously expressed transcription factors that are normally inactive but are activated in a cell experiencing a stress. Mutant heat shock factors that activate the stress protein response in the absence of a stress or that prevent stress activation of the response have proven useful for studying biological questions associated with a normal or abnormal stress protein response and have been incorporated in the design of gene switches with desirable new properties.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • Apoptosis
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Genes, Dominant*
  • Heat Shock Transcription Factors
  • Heat-Shock Proteins / genetics*
  • Heat-Shock Proteins / metabolism
  • Heat-Shock Proteins / physiology*
  • Heat-Shock Response
  • Hot Temperature
  • Humans
  • JNK Mitogen-Activated Protein Kinases
  • Mutation
  • Promoter Regions, Genetic
  • Protein Structure, Tertiary
  • RNA / metabolism
  • Structure-Activity Relationship
  • Transcription Factors
  • Transcription, Genetic
  • p38 Mitogen-Activated Protein Kinases


  • DNA-Binding Proteins
  • Heat Shock Transcription Factors
  • Heat-Shock Proteins
  • Transcription Factors
  • RNA
  • JNK Mitogen-Activated Protein Kinases
  • p38 Mitogen-Activated Protein Kinases