Nitric oxide inhibits the replication cycle of severe acute respiratory syndrome coronavirus

J Virol. 2005 Feb;79(3):1966-9. doi: 10.1128/JVI.79.3.1966-1969.2005.

Abstract

Nitric oxide (NO) is an important signaling molecule between cells which has been shown to have an inhibitory effect on some virus infections. The purpose of this study was to examine whether NO inhibits the replication cycle of the severe acute respiratory syndrome coronavirus (SARS CoV) in vitro. We found that an organic NO donor, S-nitroso-N-acetylpenicillamine, significantly inhibited the replication cycle of SARS CoV in a concentration-dependent manner. We also show here that NO inhibits viral protein and RNA synthesis. Furthermore, we demonstrate that NO generated by inducible nitric oxide synthase, an enzyme that produces NO, inhibits the SARS CoV replication cycle.

MeSH terms

  • Animals
  • Chlorocebus aethiops
  • Humans
  • Nitric Oxide / metabolism
  • Nitric Oxide / pharmacology*
  • Nitric Oxide Synthase / metabolism
  • Nitric Oxide Synthase Type II
  • S-Nitroso-N-Acetylpenicillamine / pharmacology*
  • SARS Virus / drug effects*
  • SARS Virus / physiology
  • Vero Cells
  • Virus Replication / drug effects*

Substances

  • Nitric Oxide
  • S-Nitroso-N-Acetylpenicillamine
  • NOS2 protein, human
  • Nitric Oxide Synthase
  • Nitric Oxide Synthase Type II