Tumor cells as cellular vehicles to deliver gene therapies to metastatic tumors

Cancer Gene Ther. 2005 Apr;12(4):341-9. doi: 10.1038/sj.cgt.7700801.

Abstract

A long-pursued goal in cancer treatment is to deliver a therapy specifically to metastases. As a result of the disseminated nature of the metastatic disease, carrying the therapeutic agent to the sites of tumor growth represents a major step for success. We hypothesized that tumor cells injected intravenously (i.v.) into an animal with metastases would respond to many of the factors driving the metastatic process, and would target metastases. Using a model of spontaneous metastases, we report here that i.v. injected tumor cells localized on metastatic lesions. Based on this fact, we used genetically transduced tumor cells for tumor targeting of anticancer agents such as a suicide gene or an oncolytic virus, with evident antitumoral effect and negligible systemic toxicity. Therefore, autologous tumor cells may be used as cellular vehicles for systemic delivery of anticancer therapies to metastatic tumors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bystander Effect
  • Cell Line, Tumor
  • Combined Modality Therapy / methods
  • Gene Transfer Techniques*
  • Genetic Therapy / methods*
  • Genetic Vectors
  • Green Fluorescent Proteins / metabolism
  • Humans
  • Immunohistochemistry
  • Lung / pathology
  • Lung Neoplasms / pathology
  • Lung Neoplasms / therapy
  • Mice
  • Mice, SCID
  • Neoplasm Metastasis
  • Neoplasm Transplantation
  • Neoplasms / therapy*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tissue Distribution
  • Viruses / genetics

Substances

  • Green Fluorescent Proteins