Expression of interleukin-8 gene in radical prostatectomy specimens is associated with advanced pathologic stage

Prostate. 2005 Jun 15;64(1):40-9. doi: 10.1002/pros.20223.


Background: We previously reported that relative expression of E-cadherin, matrix metalloproteinases (MMPs)-2 and -9, and vascular endothelial growth factor (VEGF)/vascular permeability factor in radical prostatectomy specimens (RP) can distinguish organ-confined cancers from advanced prostate cancers. Here, we evaluate the expression of interleukin-8 (IL-8) and basic fibroblast growth factor (bFGF), two other genes involved in angiogenesis and metastasis, in RP specimens.

Methods: The expression level of IL-8 and bFGF mRNA in the invasive edge of 41 prostate cancers of different stages was determined using a rapid colorimetric in situ hybridization (ISH) technique. Gene expression levels of IL-8 and bFGF were correlated with the Gleason score and pathologic stage to ascertain their relationship to prostate cancer progression.

Results: The expression of IL-8 and bFGF genes was detected by ISH in histologically normal prostate gland epithelium as well as in glands with foci of cancer. Increased mRNA expression of IL-8 was associated with both the Gleason score and pathologic stage of tumors and distinguished organ-confined from non-confined tumors (P = 0.002). In contrast, the expression of bFGF mRNA did not correlate with the Gleason score or pathologic stage.

Conclusions: Overexpression of Il-8 mRNA, but not bFGF mRNA, in RP specimens is directly associated with progression of prostate cancer.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Biomarkers, Tumor*
  • Fibroblast Growth Factor 2 / genetics
  • Gene Expression Regulation, Neoplastic
  • Humans
  • In Situ Hybridization
  • Interleukin-8 / genetics*
  • Male
  • Prognosis
  • Prostatectomy
  • Prostatic Neoplasms / pathology*
  • Prostatic Neoplasms / physiopathology*
  • Prostatic Neoplasms / surgery
  • RNA, Messenger / analysis


  • Biomarkers, Tumor
  • Interleukin-8
  • RNA, Messenger
  • Fibroblast Growth Factor 2