Abstract
Cannabidiol is the main nonpsychoactive component of marijuana. We examined the ability of in vivo and in vitro cannabidiol to interfere with the production of interleukin (IL)-12 and IL-10 by murine macrophages and to modulate macrophage chemotaxis. Cannabidiol added in vitro to peritoneal macrophages significantly increased IL-12 and decreased IL-10 production. The CB1 and CB2 receptor antagonists prevented this modulation. Macrophages from animals treated with cannabidiol at the dose of 30 mg kg(-1) either orally or i.p. produced higher levels of IL-12 and lower levels of IL-10 in comparison to controls, and the CB receptor antagonists did not prevent these effects. Cannabidiol dose-dependently decreased fMLP-induced chemotaxis of macrophages, and the CB2 receptor antagonist prevented this decrease.
MeSH terms
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Adjuvants, Immunologic / administration & dosage*
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Adjuvants, Immunologic / metabolism
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Adjuvants, Immunologic / pharmacology*
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Administration, Oral
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Animals
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Camphanes / pharmacology
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Cannabidiol / administration & dosage*
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Cannabidiol / metabolism
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Cannabidiol / pharmacology*
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Cell Migration Inhibition
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Cells, Cultured
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Chemotaxis / drug effects*
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Chemotaxis / immunology
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Cytokines / biosynthesis
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Down-Regulation / drug effects
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Down-Regulation / immunology
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Interleukin-10 / antagonists & inhibitors
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Interleukin-10 / biosynthesis*
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Interleukin-12 / biosynthesis*
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Macrophage Activation / drug effects
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Macrophage Activation / immunology
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Macrophages, Peritoneal / drug effects*
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Macrophages, Peritoneal / immunology
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Macrophages, Peritoneal / metabolism
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Male
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Mice
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Piperidines / pharmacology
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Pyrazoles / pharmacology
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Receptor, Cannabinoid, CB1 / antagonists & inhibitors
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Receptor, Cannabinoid, CB1 / physiology
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Receptor, Cannabinoid, CB2 / antagonists & inhibitors
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Receptor, Cannabinoid, CB2 / physiology
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Rimonabant
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Up-Regulation / drug effects
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Up-Regulation / immunology
Substances
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Adjuvants, Immunologic
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Camphanes
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Cytokines
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Piperidines
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Pyrazoles
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Receptor, Cannabinoid, CB1
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Receptor, Cannabinoid, CB2
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SR 144528
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Interleukin-10
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Interleukin-12
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Cannabidiol
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Rimonabant