The pro and the active form of matrix metalloproteinase-9 is increased in serum of patients with amyotrophic lateral sclerosis

J Neuroimmunol. 2005 Feb;159(1-2):146-54. doi: 10.1016/j.jneuroim.2004.09.015. Epub 2004 Nov 4.


Pro and active-matrix metalloproteinase-9 (MMP-9) was measured in sera from patients with amyotrophic lateral sclerosis (ALS), Guillain-Barre syndome (GBS), and healthy subjects. Both forms of MMP-9 were elevated in sera of ALS and GBS patients, compared with healthy controls. It has been postulated that elevated MMP-9 reflects damage to peripheral nerve and muscle. This possibility was investigated in sera, and tissue extracts of sciatic nerves and muscle from mice 5 and 12 days after axotomy of the sciatic nerve. Pro-MMP-9 was elevated in sera and extracts of damaged nerve and muscle, suggesting such damage may be followed by elevated pro-MM9-9 in sera. Active MMP-9 was only elevated in the sera. However, in situ activation of MMP-9 is tightly regulated and localised, and probably difficult to demonstrate by ELISA, resulting in a short half-life active MMP-9, implying any active MMP-9 in the serum may have a more immediate origin than injured muscle or nerve, for example circulating blood cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Amyotrophic Lateral Sclerosis / blood
  • Amyotrophic Lateral Sclerosis / enzymology*
  • Amyotrophic Lateral Sclerosis / pathology
  • Animals
  • Blotting, Western
  • Enzyme Activation
  • Enzyme Precursors / biosynthesis*
  • Enzyme Precursors / blood*
  • Enzyme Precursors / isolation & purification
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Guillain-Barre Syndrome / blood
  • Guillain-Barre Syndrome / enzymology
  • Humans
  • Male
  • Matrix Metalloproteinase 9 / biosynthesis*
  • Matrix Metalloproteinase 9 / blood*
  • Matrix Metalloproteinase 9 / isolation & purification
  • Mice
  • Mice, Inbred BALB C
  • Middle Aged
  • Muscle, Skeletal / enzymology
  • Muscle, Skeletal / innervation
  • Muscle, Skeletal / pathology
  • Nerve Crush
  • Sciatic Nerve / enzymology
  • Sciatic Nerve / pathology
  • Up-Regulation


  • Enzyme Precursors
  • Matrix Metalloproteinase 9