Spying on Cancer: Molecular Imaging in Vivo With Genetically Encoded Reporters

Cancer Cell. 2005 Jan;7(1):5-15. doi: 10.1016/j.ccr.2004.12.011.

Abstract

Genetically encoded imaging reporters introduced into cells and transgenic animals enable noninvasive, longitudinal studies of dynamic biological processes in vivo. The most common reporters include firefly luciferase (bioluminescence imaging), green fluorescence protein (fluorescence imaging), herpes simplex virus-1 thymidine kinase (positron emission tomography), and variants with enhanced spectral and kinetic properties. When cloned into promoter/enhancer sequences or engineered into fusion proteins, imaging reporters allow transcriptional regulation, signal transduction, protein-protein interactions, oncogenic transformation, cell trafficking, and targeted drug action to be spatiotemporally resolved in vivo. Spying on cancer with genetically encoded imaging reporters provides insight into cancer-specific molecular machinery within the context of the whole animal.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Caspase 3
  • Caspases / genetics
  • Caspases / metabolism
  • Cell Transformation, Neoplastic
  • Diagnostic Imaging*
  • Drug Resistance, Multiple / physiology
  • Gene Expression Regulation
  • Genes, Reporter*
  • Humans
  • Molecular Probe Techniques*
  • Neoplasms* / genetics
  • Neoplasms* / metabolism
  • Promoter Regions, Genetic
  • Proteasome Endopeptidase Complex / metabolism
  • RNA Processing, Post-Transcriptional
  • Transcription, Genetic

Substances

  • CASP3 protein, human
  • Caspase 3
  • Caspases
  • Proteasome Endopeptidase Complex