Proteomics analysis of human cerebrospinal fluid

J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Feb 5;815(1-2):179-89. doi: 10.1016/j.jchromb.2004.06.044.


Cerebrospinal fluid (CSF) is secreted from several different central nervous system (CNS) structures, and any changes in the CSF composition will accurately reflect pathological processes. Proteomics offers a comprehensive bird's eye view to analyze CSF proteins at a systems level. This paper reviews the variety of analytical methods that have been used for proteomics analysis of CSF, including sample preparation, two-dimensional liquid and gel electrophoresis, mass spectrometry, bioinformatics, and non-gel methods. The differentially expressed CSF proteins that have been identified by proteomics methods are discussed.

Publication types

  • Comparative Study
  • Review

MeSH terms

  • Cerebrospinal Fluid Proteins* / chemistry
  • Cerebrospinal Fluid Proteins* / genetics
  • Chromatography, Liquid
  • Databases, Protein
  • Electrophoresis, Gel, Two-Dimensional
  • Humans
  • Isoelectric Focusing
  • Mass Spectrometry
  • Proteome*
  • Proteomics / methods*
  • Spectrometry, Mass, Electrospray Ionization
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization


  • Cerebrospinal Fluid Proteins
  • Proteome