Identification and subcellular localization of two solanesyl diphosphate synthases from Arabidopsis thaliana

Plant Cell Physiol. 2004 Dec;45(12):1882-8. doi: 10.1093/pcp/pch211.

Abstract

Two solanesyl diphosphate synthases, designated SPS1 and SPS2, which are responsible for the synthesis of the isoprenoid side chain of either plastoquinone or ubiquinone in Arabidopsis thaliana, were identified. Heterologous expression of either SPS1 or SPS2 allowed the generation of UQ-9 in a decaprenyl diphosphate synthase-defective strain of fission yeast and also in wild-type Escherichia coli. SPS1-GFP was found to localize in the ER while SPS2-GFP localized in the plastid of tobacco BY-2 cells. These two different subcellular localizations are thought to be the reflection of their roles in solanesyl diphosphate synthesis in two different parts: presumably SPS1 and SPS2 for the side chains of ubiquinone and plastoquinone, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkyl and Aryl Transferases / genetics
  • Alkyl and Aryl Transferases / isolation & purification
  • Alkyl and Aryl Transferases / metabolism*
  • Arabidopsis / enzymology*
  • Arabidopsis / genetics
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / isolation & purification
  • Arabidopsis Proteins / metabolism*
  • DNA, Complementary / analysis
  • DNA, Complementary / genetics
  • Endoplasmic Reticulum / metabolism
  • Escherichia / metabolism
  • Molecular Sequence Data
  • Plastids / metabolism
  • Plastoquinone / metabolism
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Terpenes / metabolism
  • Tobacco / metabolism
  • Ubiquinone / metabolism
  • Yeasts / metabolism

Substances

  • Arabidopsis Proteins
  • DNA, Complementary
  • Terpenes
  • Ubiquinone
  • Alkyl and Aryl Transferases
  • decaprenyl pyrophosphate synthetase
  • trans-octaprenyltranstransferase
  • SPS1 protein, Arabidopsis
  • SPS2 protein, Arabidopsis
  • ubiquinone 9
  • Plastoquinone