Lactobacillus delbrueckii subsp. indicus subsp. nov., isolated from Indian dairy products

Int J Syst Evol Microbiol. 2005 Jan;55(Pt 1):401-404. doi: 10.1099/ijs.0.63067-0.


Four strains isolated from Indian dairy products and initially identified as Lactobacillus delbrueckii could not be assigned to a definite subspecies because molecular identification and phenotypic traits did not agree with those of recognized subspecies of L. delbrueckii. Hybridization of total DNA (78-86 % against type strains of the other three subspecies), AFLP and RAPD-PCR fingerprints, phylogenetic analysis based on 16S rRNA gene sequences and sequence analysis of two coding genes (recA and hsp60), together with phenotypic profiles, indicated that the four strains form a coherent cluster and represent a novel subspecies, for which the name Lactobacillus delbrueckii subsp. indicus subsp. nov. is proposed. The type strain is NCC725T (=LMG 22083T=DSM 15996T).

MeSH terms

  • Animals
  • Chaperonin 60 / genetics
  • DNA Fingerprinting / methods
  • DNA, Bacterial / analysis
  • Dairy Products / microbiology*
  • Genes, rRNA
  • India
  • Lactobacillus delbrueckii / classification*
  • Lactobacillus delbrueckii / genetics
  • Lactobacillus delbrueckii / isolation & purification
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Phylogeny
  • Polymerase Chain Reaction / methods
  • Polymorphism, Restriction Fragment Length
  • RNA, Ribosomal, 16S / genetics
  • Rec A Recombinases / genetics
  • Sequence Analysis, DNA


  • Chaperonin 60
  • DNA, Bacterial
  • RNA, Ribosomal, 16S
  • Rec A Recombinases

Associated data

  • GENBANK/AJ586863
  • GENBANK/AJ586864
  • GENBANK/AJ586865
  • GENBANK/AJ586866
  • GENBANK/AJ586867
  • GENBANK/AJ586868
  • GENBANK/AJ586869
  • GENBANK/AJ586870
  • GENBANK/AJ586871
  • GENBANK/AJ586872