Transforming growth factor-beta and its effect on reepithelialization of partial-thickness ear wounds in transgenic mice

Wound Repair Regen. Jan-Feb 2005;13(1):61-7. doi: 10.1111/j.1067-1927.2005.130108.x.


Transforming growth factor-beta (TGF-beta) is known to affect nearly every aspect of wound repair. Many of the effects have been extensively investigated; however, the primary effect of endogenously derived TGF-beta on wound reepithelialization is still not completely understood. To examine this, two types of wounds were made on a transgenic mouse over-expressing TGF-beta1. Full-thickness back wounds were made to compare the wound healing process in the presence of compensatory healing mechanisms. Superficial partial-thickness ear wounds involving only the epidermis were made to determine the effect of TGF-beta on reepithelialization. In the partial-thickness ear wounds, at later time points, the transgenic group had smaller epithelial gaps than the wild-type mice. A greater number of actively proliferating cells, as determined by bromodeoxyuridine incorporation, was also found in the transgenic mice at post-injury day 8. These results show that TGF-beta1 stimulates the rate of reepithelialization at later time points in partial-thickness wounds. However, in the full-thickness back wounds, the transgenic animals exhibited a slower reepithelialization rate at all time points and the number of bromodeoxyuridine-positive cells was fewer. Our findings would suggest that the overexpression of TGF-beta1 speeds the rate of wound closure in partial-thickness wounds by promoting keratinocyte migration. In full-thickness wounds, however, the overexpression of TGF-beta1 slows the rate of wound reepithelialization.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Back
  • Cell Movement / physiology
  • Ear
  • Epithelium / physiopathology*
  • Keratinocytes / physiology
  • Mice
  • Mice, Transgenic
  • Models, Animal
  • Transforming Growth Factor beta / physiology*
  • Wound Healing / physiology*
  • Wounds and Injuries / physiopathology


  • Transforming Growth Factor beta