Quantitative RT-PCR of Wilms tumor gene transcripts (WT1) for the molecular monitoring of patients with accelerated phase bcr/abl + CML

Leuk Res. 2005 Mar;29(3):343-5. doi: 10.1016/j.leukres.2004.08.003.

Abstract

The tyrosine kinase inhibitor imatinib inhibits the activity of the bcr/abl fusion protein present in patients with chronic myeloid leukemia. Although in chronic phase patients response to therapy can be monitored by quantitative RT-PCR for bcr/abl mRNA transcripts, in advanced disease (accelerated phase or blast crisis) only few patients respond on a molecular level. We investigated Wilms tumor gene (WT1) and bcr/abl mRNA transcripts in 16 accelerated phase CML patients by quantitative real time PCR. In contrast to the bcr/abl mRNA levels the WT1 mRNA levels were indicative for hematologic relapse (n = 6) versus response (n = 10).

MeSH terms

  • Antineoplastic Agents / therapeutic use
  • Benzamides
  • Biomarkers, Tumor / analysis*
  • Clinical Trials as Topic
  • Fusion Proteins, bcr-abl / genetics*
  • Humans
  • Imatinib Mesylate
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / drug therapy
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics*
  • Piperazines / therapeutic use
  • Prognosis
  • Pyrimidines / therapeutic use
  • RNA, Messenger / analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • WT1 Proteins / genetics*

Substances

  • Antineoplastic Agents
  • Benzamides
  • Biomarkers, Tumor
  • Piperazines
  • Pyrimidines
  • RNA, Messenger
  • WT1 Proteins
  • Imatinib Mesylate
  • Fusion Proteins, bcr-abl