RNAi as a tool to study cell biology: building the genome-phenome bridge

Curr Opin Cell Biol. 2005 Feb;17(1):3-8. doi: 10.1016/j.ceb.2004.12.008.


In the few short years since its discovery, RNA interference (RNAi) has revolutionized the functional analysis of genomes: both technical and conceptual approaches to the investigation of gene function are being transformed as a result of this new technology. Genome-scale RNAi analyses have already been performed in the model organisms Caenorhabditis elegans (in vivo) and Drosophila melanogaster (in cell lines), ushering in a new era of RNAi-based approaches to probing the inner workings of the cell. The transformation of complex phenotypic data into mineable 'digitized' formats is fostering the emergence of a new area of bioinformatics related to the phenome.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • Biology / methods*
  • Caenorhabditis elegans
  • Cell Biology
  • Drosophila melanogaster
  • Genetic Techniques*
  • Genome*
  • Humans
  • Phenotype
  • RNA Interference*
  • Software