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. 2004 Dec;10(12):2127-34.
doi: 10.3201/eid1012.040295.

Identifying Rodent Hantavirus Reservoirs, Brazil

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Free PMC article

Identifying Rodent Hantavirus Reservoirs, Brazil

Akemi Suzuki et al. Emerg Infect Dis. .
Free PMC article

Abstract

We describe the genetic analysis of samples from hantavirus pulmonary syndrome (HPS) patients from southern and southeastern states of Brazil and rodents captured at the presumed site of infection of these patients. A total of 65 samples that were antibody-positive for Sin Nombre or Laguna Negra virus by enzyme-linked immunosorbent assay were processed by nested reverse transcription-polymerase chain reaction (RT-PCR) by using several primer combinations in the M and S genome segments. PCR products were amplified and sequenced from samples from 11 HPS patient and 7 rodent samples. Phylogenetic analysis of nucleotide sequence differences showed the cocirculation of Araraquara and Juquitiba-like viruses, previously characterized from humans. Our genetic data indicate that Araraquara virus is associated with Bolomys lasiurus (hairy-tailed Bolo mouse) and the Juquitiba-like virus is associated with Oligoryzomys nigripes (black-footed pigmy rice rat).

Figures

Figure 1
Figure 1
Distribution of natural ecosystems in Brazil. Red triangles and black circles indicate the location of hantavirus pulmonary syndrome cases and rodent capture, which originated the studied DNA sequences, respectively.
Figure 2
Figure 2
Phylogenetic relationships among Brazilian and previously characterized hantaviruses. Maximum parsimony analysis of the nucleotide sequence of 303-nt fragment of the G2 gene was performed with the heuristic search option. Bootstrap values of >50%, obtained from 500 replicates of the analysis are shown. Abbreviations and GenBank accession numbers of the previously published sequences of the hantaviruses used in this study: Andes, AND-AF324901; Araraquara, ARA-AF307327; Bayou, BAY-L36930; Bermejo, BMJ-AF028025; Castelo dos Sonhos, CAS- AF307326; Hu39694-AF028023; Lechiguanas, LEC-AF028022; Laguna Negra, LN-AF005728; Maciel, MAC-AF028027; Oran, ORN-AF028024; Pergamino, PRG-AF028028; Prospect Hill, PH-X55129; Puumala, PUU-X61034; Sin Nombre, SN-CC74L33684; Hantaan, HTN strain 76/118-Y00386; Seoul, SEO-M34882; Black Creek Canal, BCC-L399500.
Figure 3
Figure 3
Phylogenetic relationship between newly and previously characterized Brazilian hantaviruses, using a 139-nt region of the M genomic segment G2 encoding region. Maximum parsimony analysis was performed by using the heuristic search option. Bootstrap values of >50%, obtained from 500 replicates of the analysis are shown. Abbreviations and GenBank accession numbers of the previously published sequences of the hantaviruses used in this study: Araraquara-AF307327 and Castelo dos Sonhos-AF307326; Juquitiba, JUQ (19).
Figure 4
Figure 4
Phylogenetic relationships between Brazilian and previously characterized hantaviruses. Abbreviations and GenBank accession numbers of the previously published sequences of the hantaviruses used in this study are listed in the legend of Figure 2. Maximum parsimony analysis of the nucleotide sequence of the 1,239-nt fragment of the M segment with the heuristic search option. Bootstrap values of >50%, obtained from 500 replicates of the analysis are shown.

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